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TLC in the experimental preparation of future chemistry teachers
| Citace: |
RICHTR, V.; VÁŇOVÁ, A.; ŽEMLIČKA, M. TLC in the experimental preparation of future chemistry teachers. Kraków, 2023.DidSci 2023.
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| Druh: | PŘEDNÁŠKA, POSTER |
| Jazyk publikace: | eng |
| Anglický název: | TLC in the experimental preparation of future chemistry teachers |
| Rok vydání: | 2023 |
| Místo konání: | Kraków |
| Autoři: | doc. Mgr. Václav Richtr CSc. , Mgr. Anna Váňová , Bc. Matěj Žemlička |
| Abstrakt EN: | Since the 20th century, when chromatography was discovered, it has spread to many areas and gained significant importance in chemistry education. It is a separation method used for analytical and preparative purposes. It achieves the separation of a mixture based on the differential migration of its components in a system of two phases - a stationary phase and a mobile phase. The stationary phase can be a solid substance or a liquid trapped in a solid porous material (carrier). The mobile phase consists of a liquid (liquid chromatography) or a gas (gas chromatography). Chromatography can be classified as column chromatography or planar chromatography, depending on the arrangement of the stationary phase. Planar chromatography includes paper chromatography and thin-layer chromatography. Thin-layer chromatography uses a finely granulated sorbent as the stationary phase, either freely or fixed with a suitable binder in a thin layer on a backing plate (adsorption chromatography). When a suitable material is chosen (stationary phase anchored on support), it is called partition chromatography. Thin-layer chromatography (TLC) can also use specially modified materials. Due to its simplicity, speed of execution, and relatively lower costs, it has also found its place in chemistry education. Future chemistry teachers should acquire both theoretical knowledge and practical skills related to this method. TLC is included in the regular teaching (laboratory exercises in organic chemistry) at the Department of Chemistry at the University of West Bohemia in Pilsen and is an important component in processing certain qualification works. Currently, it is used as both an analytical and preparative method. For analytical chromatography, commercially supplied chromatographic material or custom-made plates are used. For preparative chromatography, the chromatographic layers are prepared in the laboratory. As the work with small quantities of substances is gaining importance, preparative TLC is also becoming significant, as it can be developed in several stages as follows: 1. Preparation of the adsorption layer 2. Application of the separated material 3. Development of the chromatogram 4. Detection and isolation of components |
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